concanavalin a alexa fluor 488 (Thermo Fisher)

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Thermo Fisher concanavalin a alexa fluor 488
Concanavalin A Alexa Fluor 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alexa+488/Concanavalin+A/pmc13207355-38-0-6
Average 98 stars, based on 1 article reviews

concanavalin a alexa fluor 488 - by Bioz Stars, 2026-09

98/100 stars

Images

Staining:

Article Title: Murine nasal-associated lymphoid tissue (NALT) harbors human alphaherpesvirus 1 (HSV-1) DNA during latency, and dexamethasone triggers viral replication
Article Snippet: The cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature, washed, and stained with antibodies against ICP0 (Abcam, ab6513, 1:500), ICP4 (Abcam, ab6514, 1:500), or NeuN (NovusBio, NBP1-92693SS, 1:500) for 1 h at room temperature. .. Cells were washed and stained with secondary antibody (Alexa-488, Invitrogen A-11029; 1:1000) or Alexa-594 (Invitrogen A-11005, 1:1000) for 1 h at room temperature, washed and stained with DAPI (4′,6-diamidino-2-phenylindole, dihydrochloride, ThermoFisher Scientific) for 10 min at room temperature prior to mounting of coverslips. .. Images were obtained with an Agilent BioTek Cytation 5 and Gen5 software ( ) or an Olympus BX microscope and CellSense Entry software ( ).

Article Title: Murine nasal-associated lymphoid tissue (NALT) harbors human alphaherpesvirus 1 (HSV-1) DNA during latency, and dexamethasone triggers viral replication.
Article Snippet: The cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature, washed, and stained with antibodies against ICP0 (Abcam, ab6513, 1:500), ICP4 (Abcam, ab6514, 1:500), or NeuN (NovusBio, NBP1-92693SS, 1:500) for 1 h at room temperature. .. Cells were washed and stained with secondary antibody (Alexa-488, Invitrogen A-11029; 1:1000) or Alexa-594 (Invitrogen A-11005, 1:1000) for 1 h at room temperature, washed and stained with DAPI (4′,6-diamidino-2-phenylin dole, dihydrochloride, ThermoFisher Scientific) for 10 min at room temperature prior to mounting of coverslips. .. Images were obtained with an Agilent BioTek Cytation 5 and Gen5 software (Fig. 6) or an Olympus BX microscope and CellSense Entry software (Fig. 10).

Injection:

Article Title: Qualitative and quantitative analysis of the callosal projections to prefrontal, frontal motor, and parietal areas in the macaque monkey.
Article Snippet: .. Tracer injections and histological procedures Once the appropriate injection site was chosen, the neural tracers Fast Blue (FB, 3% in distilled water, Dr Illing Plastics GmbH, Breuberg, Germany), Diamidino Yellow (DY, 2% in 0.2 M phosphate buffer at pH 7.2, Dr Illing Plastics), True Blue (TB, 5% in distilled water, EMS-POLYLOY GmbH, Gross-Umstadt, Germany), Dextran conjugated with tetramethylrhodamine (Fluoro-Ruby, FR, 10% 0.1 M phosphate buffer, pH 7.4; Invitrogen), Cholera Toxin B subunit conjugated with Alexa 488 (CTB-green, CTBg, 1% in 0.01 m phosphate-buffered saline, pH 7.4, Invitrogen, Thermo Fisher Scientific), were slowly pressure-injected through a glass micropipette (tip diameter: 50–100 μm) attached to a 1-, 5-, or 10-μL Hamilton microsyringe, positioned with a stereotaxic holder. ..

Article Title: Qualitative and quantitative analysis of the callosal projections to prefrontal, frontal motor, and parietal areas in the macaque monkey
Article Snippet: .. Once the appropriate injection site was chosen, the neural tracers Fast Blue (FB, 3% in distilled water, Dr Illing Plastics GmbH, Breuberg, Germany), Diamidino Yellow (DY, 2% in 0.2 M phosphate buffer at pH 7.2, Dr Illing Plastics), True Blue (TB, 5% in distilled water, EMS-POLYLOY GmbH, Gross-Umstadt, Germany), Dextran conjugated with tetramethylrhodamine (Fluoro-Ruby, FR, 10% 0.1 M phosphate buffer, pH 7.4; Invitrogen), Cholera Toxin B subunit conjugated with Alexa 488 (CTB-green, CTBg, 1% in 0.01 m phosphate-buffered saline, pH 7.4, Invitrogen, Thermo Fisher Scientific), were slowly pressure-injected through a glass micropipette (tip diameter: 50–100 μm) attached to a 1-, 5-, or 10-μL Hamilton microsyringe, positioned with a stereotaxic holder. ..

Saline:

Microscopy:

Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
Article Snippet: .. Coronal sections (200μm) of lightly PFA fixed tissue were cut on a vibratome in ice-cold PBS and stored in 4°C PBS for up to 48 h. Slices were placed in RT PBS on the stage of a Zeiss microscope, and pyramidal neurons in VC L2/3 identified and soma impaled with a sharp micropipette (100–400 MΩ) backfilled with 10mM Alexa 488 (Thermo Fisher A10436) in 200 mM KCl. ..

other:

Article Title: AAV2-driven endothelin induces chronic reduced retinal blood flow/retinal ganglion cell loss in rats
Article Snippet: Alexa 488 , Rabbit , 1:500 , Thermo Fisher Scientific (A11034).

Incubation:

Article Title: Targeting GL-Lect driven endocytosis to suppress cell plasticity in breast cancer
Article Snippet: .. Cells were fixed for 10 min with PFA 4% at RT, permeabilized with Triton X100 0.1%, followed by incubation with secondary Antibody (Alexa-488, Thermofisher 5 μg/ml, see Table S5) for 30 min to visualize internalized ECAD/CD44, and DAPI counterstained. ..

Article Title: Kindlin‐2 Deletion in Mural Cells Leads to Vascular Instability
Article Snippet: Tissues were blocked with 4% horse serum and stained with rabbit anti- ACTA2 (1:100) (Abcam, Cambridge, UK), mouse anti- K2, rat anti- mouse CD31 (BD Biosciences, Franklin Lakes, NJ, USA), rabbit anti- Fg (Agilent Dako, Glostrup, Denmark), mouse anti- GFP (clone B2, Santa Cruz Biotechnology, Dallas, TX, USA) for 16 h at 4°C. .. Next, the slides were incubated with goat anti- rabbit, anti- mouse, or anti- rat Abs conjugated to Alexa 488 or Alexa 568 (ThermoFisher Scientific, Oakwood, OH, USA) for 1 h at RT. ..

Review